Senior Discovery Scientist, Synthetic Biology
South San Francisco, CA
About Pattern Biosciences (www.patternbio.com)
Do you want to be at the forefront of a revolution in healthcare? At Pattern Biosciences, we build DNA computers that run molecular programs in cells to treat cancer and other diseases. Using multi-input molecular-level computation in individual cells, our approach leads to curative therapies where the conventional one drug-one target paradigm has failed to deliver effective treatments. Our headquarters are in South San Francisco, and we are building a world-class team to translate two decades of progress in DNA computing and synthetic biology into the clinic.
About the Role
Are you passionate about working with cutting-edge technologies to develop next generation therapies? You will advance the pipeline of innovative cancer therapies harnessing our biomolecular computing platform. You will engineer genetic scaffolds to compute across multiple molecular inputs inside cells to program cancer cells for destruction with exquisite specificity. You will focus on individual sensor components, molecular computing scaffolds, and fully assembled therapeutic leads, as standalone DNA constructs and as genetic payloads of viral and non-viral delivery vectors.
What you'll do
- Design and construct barcoded genetic component libraries, logic scaffolds, effectors, and product leads
- Plan and execute in vitro and in vivo experiments to generate informative and clinically-translatable datasets to drive the design-build-test cycle of components and fully-assembled therapeutic leads
- Characterize component libraries and individual therapeutic candidates as naked and vectorized DNA in mammalian cell culture and in in vivo specimens using next-generation sequencing, fluorescent microscopy, flow cytometry, qPCR, and plate reader assays
What you'll bring
- Experience in the design, construction, and testing of gene circuits in mammalian cells
- Excellent knowledge of gene regulatory and gene expression processes and mechanisms in human cells, and the ability to translate this knowledge into actionable genetic designs
- Deep knowledge in experimental planning and execution of high-throughput cloning of barcoded libraries and individual genetic constructs using “Golden Gate”, Gibson assembly, yeast homologous recombination, restriction-ligation
- Experience in mammalian cell culture, fluorescent microscopy, flow cytometry, cell sorting, plate reader assays including ELISA, and RT-qPCR assays
- Hands-on experience in library preparation for next-generation sequencing
- Experience working with Adeno-Associated virus (AAV) vectors is an advantage
Who You Are
- PhD degree in life sciences including genetics, cancer biology, bioengineering and related fields
- 1-2 years of industry and/or postdoctoral research experience
- Track record demonstrating creativity and passion for science and patient care
- Desire and ability to operate in a high growth environment and deliver results within specified timelines and budgets
This is an in person role based at our headquarters in South San Francisco
To apply, please send a cover letter and a CV to careers@patternbio.com